USDA’s Regional Climate Hubs were established in February of 2014 to deliver science-based knowledge, practical information, and program support to farmers, ranchers, forest landowners, and resource managers to support climate-informed decision-making in light of the increased risks and vulnerabilities associated with a changing climate. As part of their function, the Hubs were tasked with providing periodic regional assessments of risk and vulnerability to production sectors and rural economies, building on material provided under the National Climate Assessment conducted through the United States Global Change Research Program (USGCRP). With the publication of this Vulnerability Assessment, the Midwest and Northwest Regional Climate Hubs are providing their stakeholders with an introduction to the region, regional sensitivities and adaptation strategies for working lands, a greenhouse gas emissions profile with mitigation opportunities, and an overview of how partner USDA agencies are being affected by a changing climate. This vulnerability assessment is an important first step in establishing a baseline “snapshot” of current climate vulnerabilities, and provides region-specific adaptation and mitigation strategies to increase the resilience of working lands in the region.